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Objective To treat cerebrovascular stenosis with percutaneous transluminal angioplasty and stenting and analyze the problems of this method and its therapeutic effects.Methods Twenty-three patients with brain watershed infarction proven by CT or MRI because of severe cerebrovascular stenosis were investigated for their clinic features,cerebrovascular digital subtraction angiography(DSA) and neurologic status before operation and after stent placement.Results The offending arteries concerning carotid sinus(C1 segment) in 14 cases,internal carotid artery(C2 segment) in 2 cases,internal carotid artery(C5,6 segment) in 4 cases;The offending arteries involving left artery in 8 cases,right in 15 cases.The percutaneous transluminal angioplasty and stenting for all patients was successful.All patients did not suffer from TIA and stroke in 6-to 12-month follow-up.Conclusion The brain watershed infarction caused by atherosclerotic cerebrovascular stenosis was not an infrequent ischemic cerebrovascular disease.The percutaneous transluminal angioplasty and stenting is an effective treatment for carotid artery or internal carotid artery stenosis.With proper selection of the patients and meticulous technique,percutaneous transluminal angioplasty and stenting should be of safety and efficacy for stroke prevention and treatment.
ABSTRACT
This study sought to synthesize a triple helix-forming phosphorothioate oligodeoxynucleotides (TFO-ps) and assess its effects on coagulation activity of the tissue factor(TF) and TF gene expression in endothelial cells. Experiment antiparallel oligodeoxynucleotides T21GTa sequence was designed and synthesized by phosphoramidite method and decorated with all-PS linkage. The affinity of TFO and TFO-ps was determined by electrophoretic mobility shift assays(EMSA). Cellular uptake of [32]P-labeled TFO-ps and the effect of TFO-ps on TF gene expression and coagulation activity of TF were measured in endothelial cell strain ECV304. The results showed that TFO-ps (T21GTa-ps) formed a triplex binding in antiparallel orientation to the puring-rich target strand-SSRE, with Kd value of 3.6 x 10(-10) M. The uptake rate of TFO-ps (T21GTa-ps) in ECV304 was about 11.65%. This compound mainly localized within the nucleus sediment(77.25%), significantly reduced the average OD of the mRNA expression and protein synthesis of TF gene, and obviously decreased the coagulation activity of TF. In conclusion, TFO-ps (T21GTa-ps) shows obvious anti-coagulation activity and its mechanism involves the inhibition of TF gene expression.
Subject(s)
Humans , Blood Coagulation , Cells, Cultured , Endothelium , Cell Biology , Gene Expression , Oligodeoxyribonucleotides , Pharmacology , RNA, Messenger , Thromboplastin , Genetics , PhysiologyABSTRACT
Objective To investigate the effect of lipid ultrasonic microbubble on the cellular absorption rate of antiparallel phosphorothioate triplex-forming oligonucleotide(TFO) and the expression of the tissue factor(TF) in endothelial cells.Methods The GT21-apsTFO,specific to the gene sequence of the shearing stress response element,was synthesized and the lipid ultrasonic microbubble carrying TFO was constructed.The ECV304 cells were divided into 4 groups:control group(SSRE),received shear stress only for 6h;TFO+ ultrasonic irradiation group(TFO+U),treated with 60?l TFO in final concentration of 0.2?mol/L and ultrasonic irradiation for 30s simultaneously;TFO-lipid microbubble group(TFO-M),treated with 60?l lipid microbubble loaded with TFO in final concentration of 0.2?mol/L;and TFO-lipid microbubble + ultrasonic irradiation group(TFO-M+U),treated with 60?l lipid microbubble loaded with TFO(final concentration was 0.2?mol/L) and ultrasonic irradiation for 30s simultaneously.Cells in the last 3 groups were treated with shear stress for 6h with pressure of 1.2Pa 24h after transfection.The fluorescent microscope was used to observe the cellular absorption rate of TFO.The immunofluorescence method and RT-PCR were employed to determine the protein and mRNA expressions of TF.Results The transfection efficiency of the TFO was higher in TFO-M+U group(38.83%?6.52%) than in TFO-M group(9.50%?2.88%) and TFO+U group(12.66%?3.01%,P
ABSTRACT
The brainstem ischemic injuries were produced by the occlusion of the basilar artery for 6 h in dogs. Gypenoside (150 mg ?kg-1)were intradudinally given at 3 h before the occlusion. We discovered that gypenoside alleviated the ischemic neuronal damage (histopathologically evaluated by the light and electronic microscope), ameliorated the abnormal changes of brainstem auditory evoked potentials (BAEP) (F